Staphylococcal Bi-Component Cytotoxins, Leukocidin and .GAMMA.-Hemolysin.
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چکیده
منابع مشابه
Further characterization of staphylococcal gamma-hemolysin.
It was confirmed that staphylococcal gamma-hemolysin is composed of two separate proteins (gamma-lysin components I and II) which act synergistically. The molecular weights of the two components, determined by gel filtration, are 29,000 and 26,000, respectively, and their isoelectric points, determined by isoelectric focusing, are at pH 9.8 and 9.9. Both components are susceptible to the action...
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Staphylococcus aureus is a major human pathogen associated with high mortality. The emergence of antibiotic resistance and the inability of antibiotics to counteract bacterial cytotoxins involved in the pathogenesis of S. aureus call for novel therapeutic approaches, such as passive immunization with monoclonal antibodies (mAbs). The complexity of staphylococcal pathogenesis and past failures w...
متن کاملPurification and properties of staphylococcal delta hemolysin.
Large amounts (200 mg per liter of culture supernatant fluid) of highly purified staphylococcal soluble delta hemolysin were obtained by adsorption to and selective elution from hydroxyapatite followed by exhaustive dialysis against water, concentration by polyvinylpyrrolidone or polyethylene glycol 20,000 dialysis, and a final water dialysis. No carbohydrate, phosphorus, or inactive 280-nm abs...
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Thirty-seven per cent of 126 strains of Staphylococcus aureus, when tested on sheep blood-agar with sensitivity discs containing cephalothin, carbenicillin, oxacillin, penicillin, and cycloserine, produced rings of beta-hemolysis surrounding zones of inhibition of bacterial growth. Each strain capable of producing a ring of beta-hemolysis did so with at least two of the mentioned antibiotics. N...
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Fractionation of highly purified staphylococcal delta hemolysin by sucrose density gradient centrifugation, Sephadex G-150 and Bio-Gel A-5m gel filtration, carboxymethyl cellulose chromatography, and isoelectric focusing failed to separate the hemolytic, protoplast-lysing, and spheroplast-lysing activities of the preparation. The activities were reduced to a similar extent when delta hemolysin ...
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ژورنال
عنوان ژورنال: Kagaku To Seibutsu
سال: 1998
ISSN: 0453-073X,1883-6852
DOI: 10.1271/kagakutoseibutsu1962.36.160